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gdna eraser  (TaKaRa)


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    Structured Review

    TaKaRa gdna eraser
    Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+fast+rt+reagent+kit/PrimeScript+FAST+RT+reagent+Kit+with+gDNA+Eraser/pmc13679839-232-14-17
    Average 99 stars, based on 486 article reviews
    gdna eraser - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury
    Article Snippet: Quantitative PCR was subsequently performed using TB Green Premix Ex Taq II Fast (CN830A, Takara, Japan) on a QuantStudio 5 real-time PCR system (Applied Biosystems, USA). .. First-strand cDNA was then synthesized from the extracted RNA using the PrimeScript Fast RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Quantitative PCR was subsequently performed using TB Green Premix Ex Taq II Fast (CN830A, Takara, Japan) on a QuantStudio 5 real-time PCR system (Applied Biosystems, USA).

    Article Title: Nigella sativa extract modulates Nrf2-related and renal transporter expression in a rat model of cisplatin-induced acute kidney injury
    Article Snippet: .. cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara, Japan), which includes an integrated step for the effective elimination of genomic DNA contamination, ensuring high-quality cDNA templates. .. RT-qPCR was conducted on the Rotor-Gene Q (QIAGEN, Hilden, Germany) using qPCRBIO SyGreen Mix (PCRBIO, PB20.14) in 20 μL reaction volumes containing 200 nM of appropriate primers (Table 1).

    Isolation:

    Article Title: Vicenin-2 alleviates oxidative stress in HT22 cells and Caenorhabditis elegans as a potential KEAP1-NRF2 interaction inhibitor.
    Article Snippet: Flavone C-glycosides are a class of compounds characterized by glycosyl moieties directly bonded to the flavone skeleton via C–C linkages, predominantly on the C-6 and C-8 positions of the A-ring.. Vicenin-2, a 6,8-di-C-glycoside derived from apigenin, is widely present in some dietary sources such as juice, herbal teas and stewed soups.. While its antioxidant potential is recognized, the molecular mechanisms underlying its protective effects against oxidative stress remain poorly explored.

    C.Elegans:

    Article Title: Vicenin-2 alleviates oxidative stress in HT22 cells and Caenorhabditis elegans as a potential KEAP1-NRF2 interaction inhibitor.
    Article Snippet: Flavone C-glycosides are a class of compounds characterized by glycosyl moieties directly bonded to the flavone skeleton via C–C linkages, predominantly on the C-6 and C-8 positions of the A-ring.. Vicenin-2, a 6,8-di-C-glycoside derived from apigenin, is widely present in some dietary sources such as juice, herbal teas and stewed soups.. While its antioxidant potential is recognized, the molecular mechanisms underlying its protective effects against oxidative stress remain poorly explored.

    Reverse Transcription:

    Article Title: CRISPR/Cas9-mediated knockout reveals non-redundant roles of dsRNases in RNAi efficiency in the melon fly Zeugodacus cucurbitae
    Article Snippet: RNA interference (RNAi) is a powerful tool for functional genomic research in insects and a promising avenue for developing eco-friendly biopesticides; however, its low RNAi efficiency remains a major bottleneck to widespread application.. Double-stranded RNA nucleases (dsRNases) are key regulators of insect RNAi efficiency.. In this study, we identified seven ZcdsRNase genes in the melon fly, Zeugodacus cucurbitae, a globally destructive tephritid pest.

    Article Title: Novel phorbol-12-O-octoate derivatives exert a dual effect by inhibiting de novo HIV-1 infection and reactivating latent virus.
    Article Snippet: .. EGR1 mRNA expression levels were measured after stimulating ACH2 cells with 1 μM HPB-5073 for 1 h and 24 h. Total RNA was extracted from the treated cells using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcribed using the PrimeScript Fast RT reagent Kit with gDNA Eraser (TaKaRa, Kusatsu, Japan). .. Relative gene expression levels were quantified by RT-qPCR using TB Green Premix Ex Taq or Premix Ex Taq (Probe qPCR) (TaKaRa).

    Article Title: Polyomavirus Drives Cerebellar Lesions in AIDS Monkeys With Ataxia
    Article Snippet: Genomic DNA was purified from tissues, SH‐SY5Y cells, and culture supernatants using a TIANamp Genomic DNA Kit (DP304, TIANGEN, China). .. RNA was reverse transcribed into cDNA using a PrimeScript FAST RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Nucleic acid concentrations were quantified with a NanoDrop ND2000 spectrophotometer (Thermo, USA).

    Article Title: AFF4 Loss Compromises Osteogenesis of Orofacial Mesenchymal Stem Cells During Alveolar Bone Repair via AMPK-Mediated Mitophagy.
    Article Snippet: Total RNA was isolated from OMSCs using TRIzol (Invitrogen), and its concentration was measured with a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific). .. Reverse transcription was conducted using the PrimeScript FAST RT reagent kit with gDNA Eraser (RR092A; TaKaRa). .. Subsequently, qRT- PCR was performed with TB Green Premix Ex Taq II FAST qPCR (CN830A; TaKaRa) on a Roche LightCycler 480 II system (Switzerland).

    Article Title: Fusobacterium nucleatum-induced endothelial ferroptosis promotes atherosclerosis.
    Article Snippet: Objective: This study aimed to investigate whether Fusobacterium nucleatum (Fn), a key periodontal pathogen, induces ferroptosis in vascular endothelial cells and to evaluate its role in Fn-associated atherosclerosis

    Expressing:

    Article Title: Novel phorbol-12-O-octoate derivatives exert a dual effect by inhibiting de novo HIV-1 infection and reactivating latent virus.
    Article Snippet: .. EGR1 mRNA expression levels were measured after stimulating ACH2 cells with 1 μM HPB-5073 for 1 h and 24 h. Total RNA was extracted from the treated cells using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcribed using the PrimeScript Fast RT reagent Kit with gDNA Eraser (TaKaRa, Kusatsu, Japan). .. Relative gene expression levels were quantified by RT-qPCR using TB Green Premix Ex Taq or Premix Ex Taq (Probe qPCR) (TaKaRa).



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